The NRB Mass Spectrometry and Proteomics Core provides mass spectrometry services with a strong bias towards proteomics analysis to investigators in the New Research Building at Harvard Medical School as well as the Harvard campus in the Longwood medical area. The focus of the core is a targeted proteomics approach in which proteins and/or protein complexes are purified prior to analysis by immunoprecipitation, protein-protein interactions, chromatographic, and electrophoretic methods.

The major services offered are protein identification, identification of post-translational modification sites, and relative quantification of protein expression and post-translational modification sites through stable-isotope labeling and isotope-free methods. The core has state-of-the-art instrumentation such as a ThermoFinnigan LTQ 2D linear ion trap mass spectrometer and a high resolution AB SCIEX QSTAR Pulsar i hybrid quadrupole-TOF mass spectrometer both operated in microcapillary LC/MS/MS mode at nanoliter flow-rates. The core also has a high resolution 4700 MALDI-TOF/TOF mass spectrometer. The core uses the latest software versions of Sequest Proteomics Browser and Mascot for database searching on dual processor PCs and houses a laminar flow clean hood and a warm room for enzymatic digestions.

For more information about the Core please contact John Asara.