The Harper Lab
Harvard Medical School
Department of Pathology
The cell cycle is composed of a series of transitions that culminate in the duplication of chromosomes through the process of DNA replication and the separation of chromosomes into daughter cells through the process of mitosis. Cell cycle transitions are frequently controls through ubiquitin mediated proteolysis of proteins that negatively regulate cell cycle progression. This laboratory studies the proteins and regulatory circuits that catalyze cell cycle and checkpoint dependent protein turnover using a variety of genetic, proteomic, and cell biological approaches.
The Ubiquitin-Proteasome Pathway and Cell Cycle Regulation
Sowa, M.E., Bennett, E.J., Gygi, S.P., and Harper, J.W. (2009) Defining the Human Deubiquitinating Enzyme Interaction Landscape. Cell, 138:389-403.
Svendsen, J., Smogorzewska, A.,Sowa, M.E., O’Connell, B.O., Gygi, S.P., Elledge, S.J., and Harper, J.W. (2009) Mammalian BTBD12/SLX4 assembles a Holliday junction resolvase and is required for DNA repair. Cell, 138:63-77.
Ang XL, Harper JW. A road map of cellular protein homeostasis.
Nature Chemical Biology. 2009;5:9-11.
Ciccia, A, Bredemeyer, AL, Sowa, ME, Terret, M-E, Jallepalli, PV, Harper, JW, and Elledge, SJ. (2009) The SIOD disorder protein SMARCAL1 is an RPA-interacting protein involved in replication fork restart. Genes and Development, 23:2415-2425.
Recent/Upcoming Events
We have developed a proteomics platform called CompPASS (Comparative Proteomics Analysis Software Suite) (Sowa et al., Cell, 2009). CompPASS is designed to help facilitate the identification of high confidence candidate interacting proteins from IP-MS/MS data. The CompPASS website contains all of the data from the Cell paper as well as tools for navigating this data, searching this data, and for uploading and analyzing your own data. This software can be accessed using the link above and following the directions provided there.
